Modifying Enzymes
Omega Biotek Inc Proteinase K, 10 mL solution, 20 mg/mL concentration, non-specific serine protease, RNase/DNase free, high stability in SDS, EDTA, urea
Omega Bio-tek Proteinase K is a non-specific serine protease that is essential for various molecular biology applications. Supplied as a ready-to-use 10 mL solution, it effectively inactivates nucleases and promotes cell lysis, ensuring high-quality DNA and RNA isolation. This enzyme is stable across a broad pH range and maintains its activity even in the presence of protein-denaturing agents such as SDS, EDTA, and urea.
- Volume: 10 ml
- Formulation: Ready-to-use solution
- Concentration: 20 mg/ml
- Activity: >600 mAU/mg
- Enzyme type: Non-specific serine protease
- Source organism: Tritirachium album
- Stability: High stability in SDS, EDTA, urea
- pH range: Wide range
- Primary application: Inactivation of nucleases and lysis for DNA/RNA isolation
- Purity: RNase/DNase free
- Storage temperature: -20°C
- Shipping condition: Requires ice pack
- Country of origin: USA
New England Biolabs, Inc. T4 Polynucleotide Kinase Reaction Buffer – 4 ml
New England Biolabs supplies a 10X reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of the enzyme.
New England Biolabs, Inc. GpC Methyltransferase (M.CviPI) – 1000 units
Methylates all cytosine residues (C5) within the double-stranded dinucleotide recognition sequence 5´...GC...3´.
Sigma Aldrich Fine Chemicals Biosciences Malonyl 13C3 coenzyme A li10MG
Malonyl 13C3 coenzyme A li10MG
Zymo Research Corporation DNA Degradase™ (500 U)
DNA Degradase™ is ideal for whole-genome DNA methylation analysis by a number of downstream applications (i.e., HPLC, TLC, etc.). Digestion with the enzyme is performed via a one-step procedure that is faster and simpler than other available methods.DNA Degradase™degrades DNA to individual nucleotides.Quantitation of nucleotides to detect DNA methylation levels can be assessed by various applications (e.g.- TLC, HPLC, etc.). For detection of DNA methylation via LC/MS, DNA needs to be degraded down to individual nucleosides.
Zymo Research Corporation Zymolyase Ultra 10000 U
Digestion of yeast and fungal cell walls is necessary for many experimental procedures. Lytic enzymes like Zymolyase Lyticase Glucanase or Chitinase are routinely used for digestion of cell walls. Distinguished from conventional lytic enzymes Zymolyase Ultra is a novel formulation of enzyme that is optimized for efficient yeast cell wall digestion. It contains enzymes that can degrade several different components of yeast cell walls. It is extensively purified using a novel DNA/RNA removal technology that results in extremely low nucleic acid contamination. It is suitable for qPCR and sequencing-based assays where low DNA/RNA contamination is critical.
New England Biolabs, Inc. Antarctic Thermolabile UDG – 100 units
Antarctic Thermolabile UDG (Uracil- DNA Glycosylase) catalyzes the release of free uracil from uracil-containing single-stranded or double-stranded DNA. The resulting abasic sites are susceptible to hydrolytic cleavage at elevated temperature and high pH. This enzyme is sensitive to heat and can be rapidly and completely inactivated at temperatures above 50C.
Athens Research And Technology Inc CATALASE HU ERYTHROCYTE 1MG
Catalase, Human Erythrocyte: 1 MG; Frozen in 50 mM Tris-HCl, pH 8.0; MW 244,000; Extinction Coefficient=1.39; Purity: > =95% by SDS-PAGE; Activity: > 50,000 u/mg protein. pH 7.0. Prepared from whole blood in the Athens Research & Tech lab.
Cayman Chemical Oleoyl SerotonIn 10mg
A hybrid molecule patterned after N-arachidonoyl serotonin; inhibits capsaicin-induced TRPV1 channel activation (IC50 = 2.57 μM) without blocking FAAH-mediated hydrolysis of arachidonoyl ethanolamine (IC50 > 50 μM)
New England Biolabs, Inc. α1-2 Fucosidase - 1000 units
a1-2 Fucosidase is a highly specific exoglycosidase that catalyzes the hydrolysis of linear 1-2 linked fucose residues from oligosaccharides
New England Biolabs, Inc. Taq DNA Ligase – 10000 units
Taq DNA Ligase is a thermostable ligase that catalyzes the formation of a phosphodiester bond between the 5'-phosphate and the 3'-hydroxyl of two adjacent DNA strands. The strands to be ligated need to be hybridized and accurately paired, with no gap, to a complementary DNA strand; allowing resolution of single nucleotide variants. Taq DNA Ligase uses NAD as a cofactor and it is active at elevated temperatures (37 C - 75 C).
New England Biolabs, Inc. Exonuclease T – 250 units
Exonuclease T (Exo T), also known as RNase T, is a single-stranded RNA or DNA specific nuclease that requires a free 3' terminus and removes nucleotides in the 3' to 5' direction. Exonuclease T can be used to generate blunt ends from RNA or DNA molecules that have 3 extensions.